Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSCs
cell type
iPSCs
condition
Corrected_pG_clone_50
chip antibody
CTCF, Abcam

Sequenced DNA Library

library_name
GSM6744930
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Decrosslinking from beads was performed in a 1% SDS solution containing Tris-HCl pH8 and 10 mM EDTA for 2 hours at 69°C with agitation. Decrosslinking of the DNA-protein complex was performed by adding Proteinase K (20 ng/mL) for 2 hours at 62°C with agitation. DNA was then purified using ExpinTM PCR SV (103-102, GeneAll®). ChIP-Seq libraries were generated according to Illumina's instructions and single-end (SE) sequencing was performed on the Illumina Hi-Seq 2500 system at the Technion Genome Center (Haifa, Israel)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
43957641
Reads aligned (%)
95.4
Duplicates removed (%)
49.7
Number of peaks
19751 (qval < 1E-05)

hg19

Number of total reads
43957641
Reads aligned (%)
94.7
Duplicates removed (%)
51.8
Number of peaks
19378 (qval < 1E-05)

Base call quality data from DBCLS SRA