GSM6744930: cG50 CTCF IP rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA
Attributes by original data submitter
Sample
source_name
iPSCs
cell type
iPSCs
condition
Corrected_pG_clone_50
chip antibody
CTCF, Abcam
Sequenced DNA Library
library_name
GSM6744930
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Decrosslinking from beads was performed in a 1% SDS solution containing Tris-HCl pH8 and 10 mM EDTA for 2 hours at 69°C with agitation. Decrosslinking of the DNA-protein complex was performed by adding Proteinase K (20 ng/mL) for 2 hours at 62°C with agitation. DNA was then purified using ExpinTM PCR SV (103-102, GeneAll®). ChIP-Seq libraries were generated according to Illumina's instructions and single-end (SE) sequencing was performed on the Illumina Hi-Seq 2500 system at the Technion Genome Center (Haifa, Israel)